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Baum, M., Budzar, A.U., Cuzick, J., Forbes, J., Houghton, J.H., Klijn, J.G. and Sahmoud, T. (2002) Anastrozole Alone or in Combination with Tamoxifen versus Tamoxifen Alone for Adjuvant Treatment of Postmenopausal Women with Early Breast Cancer: First Results of the ATAC Randomised Trial. The Lancet, 359, 2131-2139. http://dx.doi.org/10.1016/S0140-6736(02)09088-8

has been cited by the following article:

• TITLE: Sensitivity Evaluation of Two Human Breast Cancer Cell Lines to Tamoxifen through Apoptosis Induction

  AUTHORS: Spencer Keene, Charles Azuelos, Shyamal K. Majumdar

  KEYWORDS: Tamoxifen, Apoptosis, MCF-7 and MDA-MB-231 Human Breast Cancer Cell Lines, Mitochondrial Membrane Potential Assay, Estrogen Receptor

  JOURNAL NAME: Open Journal of Apoptosis, Vol.3 No.4, October 24, 2014

  ABSTRACT: Tamoxifen citrate (TAM) has been used to treat breast cancer in women for many years. The com-parative effects of TAM in inducing apoptosis were evaluated in estrogen receptor-positive (ER- positive MCF-7) and estrogen receptor-negative (ER-negative MDA-MB-231) human breast cancer cell lines in vitro in order to determine if these two cell lines differ in their sensitivity to TAM. Mi-tochondrial membrane permeability potential disruption was assessed in both cell lines by a lip-ophilic cationic dye (DePsipher assay, Trevigen, Inc.) utilizing fluorescence microscopy. Using this specific fluorochrome, we were able to associate mitochondrial membrane disruption to early, mid-, and late apoptotic cells. TAM induced cell death via apoptosis in both ER-positive and ER- negative cells, however, apoptosis induction was more pronounced in ER-positive MCF-7 compared to ER-negative MDA-MB-231 breast cancer cells. These findings may have some therapeutic use in the treatment of estrogen dependent and estrogen independent breast cancer.