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Goetz, M., Schaid, D., Wickerham, D., Safgren, S., Mushiroda, T., Kubo, M., Batzler, A., Costantino, J., Vogel, V.G., Paik, S., Carlson, E., Flockhart, D., Wolmark, N., Nakamura, Y., Weinshilboum, R., Ingle, J. and Ames, M. (2012) Evaluation of CYP2D6 and Efficacy of Tamoxifen and Raloxifene in Women Treated for Breast Cancer Chemoprevention: Results from the NSABP P-1 and P-2 Clinical Trials. Clinical Cancer Research, 17, 6944-6951. http://dx.doi.org/10.1158/1078-0432.CCR-11-0860

has been cited by the following article:

• TITLE: Sensitivity Evaluation of Two Human Breast Cancer Cell Lines to Tamoxifen through Apoptosis Induction

  AUTHORS: Spencer Keene, Charles Azuelos, Shyamal K. Majumdar

  KEYWORDS: Tamoxifen, Apoptosis, MCF-7 and MDA-MB-231 Human Breast Cancer Cell Lines, Mitochondrial Membrane Potential Assay, Estrogen Receptor

  JOURNAL NAME: Open Journal of Apoptosis, Vol.3 No.4, October 24, 2014

  ABSTRACT: Tamoxifen citrate (TAM) has been used to treat breast cancer in women for many years. The com-parative effects of TAM in inducing apoptosis were evaluated in estrogen receptor-positive (ER- positive MCF-7) and estrogen receptor-negative (ER-negative MDA-MB-231) human breast cancer cell lines in vitro in order to determine if these two cell lines differ in their sensitivity to TAM. Mi-tochondrial membrane permeability potential disruption was assessed in both cell lines by a lip-ophilic cationic dye (DePsipher assay, Trevigen, Inc.) utilizing fluorescence microscopy. Using this specific fluorochrome, we were able to associate mitochondrial membrane disruption to early, mid-, and late apoptotic cells. TAM induced cell death via apoptosis in both ER-positive and ER- negative cells, however, apoptosis induction was more pronounced in ER-positive MCF-7 compared to ER-negative MDA-MB-231 breast cancer cells. These findings may have some therapeutic use in the treatment of estrogen dependent and estrogen independent breast cancer.