TITLE:
Antithrombotic Effects of Different Strains of Lactic Acid Bacteria
AUTHORS:
Hiromasa Ooe, Masaru Kato, Kanae Hyodo, Keiko Nakashima, Hiroshi Ashigai, Kyoko Kato, Toshinori Sasaki, Yuko Fukushima, John C. Giddings, Junichiro Yamamoto
KEYWORDS:
Antithrombotic Lactic Acid Bacteria, Shear-Induced Thrombosis, Global Thrombosis Test (GTT), Laser-Induced Thrombosis, Endogenous Thrombolysis
JOURNAL NAME:
Health,
Vol.6 No.18,
October
16,
2014
ABSTRACT: Objective:
The aim of the present study was to assess the antithrombotic properties of
different strains of orally available antithrombotic lactic acid bacteria
(LAB). Research Methods & Procedures: Antithrombotic activity, antiplatelet
reactivity and/or thrombolytic activity, were measured in seven strains of LAB
and LAB cell-free-extracts (LAB filtrates) using the shear-induced platelet
reactivity/thrombolytic activity, The Global Thrombosis Test (GTT), with
non-anticoagulated rat blood in vitro.
Subsequently, the most potent antithrombotic strains identified in vitro were assessed in vivo after oral
administration in mouse carotid arteries using a helium-neon laser-induced
thrombosis model. Result: Five strains out of seven LAB (Lactobacillus paracasei KW 3100, Lactobacillus fermentum NBRC 3961, Lactobacillus pentosus JCM 8333, Leuconostoc oeni Elios 1, Pediococcus
pentosaceus NK-2) promoted significant endogenous thrombolysis in vitro. In addition, one strain (Lactobacillus fermentum NBRC 3961)
significantly inhibited shear-induced platelet re-activity. Three antithrombotic strains, Lactobacillus fermentum NBRC 3961, Leuconostoc oeni Elios 1, and Lactobacillus pentosus JCM 8333, were further assessed in vivo. The results demonstrated that
filtrates, and the cells per se of
these LAB, modulated antiplatelet activity and/or thrombolytic activity, and that the antithrombotic mechanisms
were mainly influenced by protein content (60% - 70% of dry matter). Conclusion:
The findings suggested that some strains of lactic acid bacteria could usefully
provide the basis for the production of oral antithrombotic probiotics.