TITLE:
Pseudomonas aeruginosa BUP2—A Novel Strain Isolated from Malabari Goat Produces Type 2 Pyoverdine
AUTHORS:
Kizhakkepowathial Nair Unni, Prakasan Priji, Valerie A. Geoffroy, Mukesh Doble, Sailas Benjamin
KEYWORDS:
Pseudomonas aeruginosa BUP2, Siderophore, Pyoverdine Type 2, Isoelectric Focusing
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.11,
October
14,
2014
ABSTRACT: This study focuses
on the isolation and characterization of a novel strain of siderophore
producing bacterium, i.e., Pseudomonas aeruginosa BUP2 (Pa BUP2) from the rumen of Malabari
goat, coupled with qualitative and quantitative analyses of the siderophore
produced by it. Pa BUP2—a facultative anaerobe was tuned to be an aerobe by
repeatedly growing in Benjamin flask. The
new isolate was grown in a specially designed semi-synthetic medium, designated
as BUP medium, and the yellowish-green
pigment produced was identified as a typical siderophore by spectrophotometry,
Chromazurol-S assay, thin layer chromatography and isolectric focusing (IEF).
The characteristic orange fluorescence upon UV irradiation on chromatogram and
absorption maximum at λ404 confirmed that the characteristic siderophore produced by Pa BUP2 was a typical pyoverdine (PVD). This PVD was further
categorized under type 2 by comparing its profile on the IEF gel with that of
the representative strains of each PVD types, viz., Pa O1, Pa ATCC 27853 and Pa6. Moreover, the type 2 PVD was
purified by XAD-4 Amberlite column chromatography and quantified; maximum yield
(11.17 mg/ml) was observed on day 4 of incubation (37°C). Thus, it was
confirmed that the bacterium isolated from the rumen content of Malabari goat
is a novel strain of Pa capable of
producing large quantity of PVD type 2 in specially designed BUP medium under
aerobic condition, and that its clinical and industrial implications remain
elusive.