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Pipes, W.O., Minnigh, H.A., Moyer, B. and Troy, M.A. (1986) Comparison of Clark’s Presence-Absence Test and the Membrane Filter Method for Coliform Detection in Potable Water Samples. Applied and Environmental Microbiology, 52, 439-443.

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• TITLE: A New Epidemiological Tool for Staphylococcus aureus Surveillance

  AUTHORS: Stephen C. Edberg, J. Michael Miller

  KEYWORDS: MRSA, Staphylococcus aureus, Methicillin-Resistant, Epidemiology

  JOURNAL NAME: Advances in Microbiology, Vol.4 No.13, October 14, 2014

  ABSTRACT: Epidemiological surveillance for microbes is currently based on either agar culture followed by identification, or genetic amplification. Both techniques are highly skilled-labor intensive, costly, and must be done in central laboratories. The Defined Substrate Utilization® (DSU®) format provides an epidemiological series of specific screening formulations that obviate these limitations. All reagents are present in optimized stable powder form in a test tube—add water, inoculate, and incubate. A specific color change provides a sensitive and specific detection of the target microbe. Two DSU® methods for Staphylococcus aureus (S. aureus) are presented: aureusAlert® for all S. aureus and EPI-M® for methicillin-resistant S. aureus (MRSA). Both aureusAlert® and EPI-M® had a detection level of 20 colony forming units (CFU) in 18 hours. aureusAlert® and conventional methods agreed 93.6% and EPI-M® and conventional methods agreed 94.1%. DSU® and conventional methods showed the same specificity.