TITLE:
Hemoglobin S Polymerization Effect on Water Self-Diffusion Coefficient
AUTHORS:
Manuel A. Lores Guevara, Juan C. García-Naranjo, Yulianela Mengana, Jorge Pereira
KEYWORDS:
Sickle Cell Disease, Water Self-Diffusion Coefficient, T2, NMR
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.4 No.6,
October
9,
2014
ABSTRACT: The transversal relaxation time, the effective transversal relaxation time and the water self-diffusion coefficient are evaluated during hemoglobin S polymerization. One homogeneous permanent magnet and one inhomogeneous and portable unilateral magnet with a very strong and constant static magnetic field gradient were utilized. The Carr-Purcell-Meiboom-Gill method was used before and after placing the studied samples 24 hours at 36°C to guarantee the polymerization. The transversal relaxation shows two exponents after polymerization supporting the concept of partially polymerized hemoglobin. The effective transversal relaxation time decreases around 40%, which can be explained by the increase of water self-diffusion coefficient 1.8 times as a main value. This result can be explained considering the effects of the agglutination process on the obstruction and hydration effects in a partially polymerized solution.