TITLE:
New Approach for Isolation of Individual Caseins from Cow Milk by the Preparative Electrophoresis
AUTHORS:
Andrii V. Iukalo
KEYWORDS:
Casein, Isolation, Individual Forms, Electrophoresis, Preparative
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.4 No.6,
October
9,
2014
ABSTRACT: This paper carried out a comparative analysis of different types of electrophoretic systems which were used for the analysis of casein complex from cow milk (polyacrylamide gel electrophoresis: for the neutral and acidic native conditions, in gradient variant, the presence of sodium dodecyl sulphate or with including urea). Taking into attention the separation efficiency, complexity of electrophoretic system, the impact of system components, we have selected the anode system of the homogeneous gel in the presence of urea as the basis for the preparation of casein fractions. It also changed the composition and the structure of the electrophoretic apparatus. The changes allow purification of casein fractions up to several grams during one stage of treatment (for 5 hours). The purified casein fractions were tested for the homogeneity and have been recommended for using in the biomedical researches, including the processes of the formation of the bioactive peptides.