TITLE:
Automatic Evaluation of Test Strips for Anti-Ganglioside Antibodies in Patients with Guillain-Barré Syndrome Using EUROLineScan Software
AUTHORS:
Chetana Vaishnavi, Chitralekha Behura, Sudesh Prabhakar
KEYWORDS:
Anti-Ganglioside Antibodies, C. Jejuni, Guillain-Barré Syndrome
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.13,
October
9,
2014
ABSTRACT: Campylobacter jejuni infection has been implicated in the pathogenesis
of Guillain-Barré syndrome (GBS) due to production of humoral immune response against neural
antigens. A case-control study was performed in a tertiary care teaching
hospital for the estimation of anti-ganglioside antibodies in GBS patients and
their controls. Blood samples were collected from 59 GBS cases, 58 neurological
controls (NC) and 60 non-neurological control (NNC) patients for automatic
estimation of IgG and IgM antibodies to seven gangliosides using EUROLineScan
software. Antibodies of IgG class for GM1 were highly significant in GBS (p = 0.000)
and NC (p = 0.031) compared to NNC. However GBS group was not significant (p = 0.413)
compared to NC. For GM2 ganglioside, GBS and NC groups were significant (p = 0.000)
compared to NNC, but GBS group was not significant (p = 0.999) compared to NC.
For GM3 ganglioside, GBS and NC groups were significant (p = 0.000) compared to
NNC; but GBS group was insignificant (p = 0.858) compared to NC with similar
trend for all other ganglioside antibodies. When IgM class of antibodies was
evaluated for GM1, GBS group was not significant (p = 0.604) whereas NC group
was significant (p = 0.000) compared to NNC. GBS group was not significant
compared to NC (p = 0.011). The trend was the same for GM2 antibodies. For GM3,
GBS group was significant (p = 0.010) and NC was near significant (p = 0.055)
compared to NNC. However GBS group was not significant (p = 0.808) compared to
NC. No groups were significant (p > 0.05) in relation to the remaining
gangliosides except for GQ1b where GBS group (p = 0.001) and NC group were
significant (p = 0.000) compared to NNC. GBS group was also significant (p = 0.001)
compared to NC and NNC. Anti-gangliosides antibodies were present in highly
significant levels in the GBS group, though they were also present in the
non-paralytic neurological control patients compared to the non-neurological
control group.