TITLE:
Green Synthesis and Characterization of Gold Nanoparticles: Study of Its Biological Mechanism in Human SUDHL-4 Cell Line
AUTHORS:
Umesh K. Parida, Susanta K. Biswal, Birendra K. Bindhani
KEYWORDS:
Green Gold, Nanomedicine, Cell Line, Apoptosis, Oxidative Stress, ROS, Caspase
JOURNAL NAME:
Advances in Biological Chemistry,
Vol.4 No.6,
October
6,
2014
ABSTRACT: In this investigation, the anticancer potentiality and biological
mechanism of gold nanoparticles (AuNPs) was studied in SUDHL-4 cell line.
Metallic AuNPs were prepared and stabilized with ethanol clove (Syzygium aromaticum) extract. The green
synthesis of AuNPs was characterized and evaluated by UV-Visible Spectroscopic,
X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR),
Transmission electron microscopy (TEM), Dynamic Light Scattering (DLS) and biological
activities using various biochemical assays. Green synthesis of AuNPs was
confirmed by instrument method. The TEM images show polydis-perse, mostly
spherical AuNPs particles of 12 - 20 nm. AuNPs were decreased the growth and viability of SU-DHL-4
cell line and increased the apoptosis. The treatments of SU-DHL-4 cells with
AuNPs resulted in a moderate considerably increase in Reactive oxygen species
(ROS) production. We measured apoptosis by Annexin-V/propidium iodide (PI) in
the existence and nonexistence of the antioxidant N-acetyl-L-cysteine (NAC),
the glutathione-depleting agent buthionine sulfoximine (BSO), or caspase
inhibitors to determine the mechanism of cell death. AuNPs are unique potential
anticancer agents that cause ROS-dependent apoptosis in SUDHL-4 cell line which
was improved by depletion of glutathione (GHS) and inhibited by
N-acetyl-L-cysteine on Z-VAD-FMK.