TITLE:
Laser Induced Fluorescence Studies of Blood Plasma and Tumor Tissue of Men with Prostate Tumors
AUTHORS:
Liana Ramishvili, Irma Bochorishvili, Nino Gabunia, Zurab Kuchukashvili, Manana Gordeziani, Teimuraz Chigogidze, Ana Khazaradze, Zaza Melikishvili, Nanuli Kotrikadze
KEYWORDS:
Prostate Tumors, Fluorescence, Endogenous Fluorophores, Energy Metabolism
JOURNAL NAME:
Journal of Cancer Therapy,
Vol.5 No.11,
September
26,
2014
ABSTRACT: Objectives: Fluorescence spectroscopy
which can be used for optical tissue diagnosis of tumor pathologydeserves
special interest. The purpose of the work was to study blood plasma and tumor
tissue of men with different forms of prostate tumors by using laser induced
fluorescence. Blood plasma and tumor tissue of the patients with benign
hyperplasia of the prostate (BHP), BHP with inflammation, BHP with high grade
PIN (BHP with HGPIN) and adenocarcinoma of prostate (CaP) have been studied.
Results: In case of blood plasma fluorescence,intensity of the plasma
proteins corresponding peak (340 - 360 nm) was increasing in the following
manner: control group → BHP → BHP with HGPIN → CaP. The intensity of the
nicotinamide coenzymes correspond peak (440 - 460 nm) was increased in case of
BHP with HGPIN and CaP patients, but decreased in case of BHP, compared to
control. In case of tumor tissue, the changes of the collagen peak (390 - 400
nm) intensity have been revealed in all cases of prostate tumor tissues. These
alterations point to altered collagen biosynthesis levels in different tumor
tissues, that reflects the structural changes and characteristics of malignant
transformation. Also the changes of the nicotinamide coenzymes peak (440 - 460
nm) intensity in all spectra of tumor tissues were observed. The highest
intensity of the peak was observed in the spectra of BHP with HGPIN and in
prostate cancer tissue. Conclusions: Alterations of the coenzymes peak
intensities perfectly reflect and are in accordance with the specific energy
metabolism of prostate epithelial cells. Normalization of fluorescent spectra
from different forms of prostate tumor tissues has shown that, each form has
typical spectral shape and ratio of fluorescence peaks intensities.