TITLE:
Isolation and Characterization of Hydrocarbon-Degrading Bacteria from Wastewaters in Ouagadougou, Burkina Faso
AUTHORS:
Adama Sawadogo, Otoidobiga C. Harmonie, Joseph B. Sawadogo, Aminata Kaboré, Alfred S. Traoré, Dayéri Dianou
KEYWORDS:
Biodegradation, Hydrocarbon, Bacteria, Wastewater, Depollution, Burkina Faso
JOURNAL NAME:
Journal of Environmental Protection,
Vol.5 No.12,
September
24,
2014
ABSTRACT: Lubricants
are very often found in nature under waste-oil forms and represent for the
environment a real danger of pollution due to the difficulty of their biodegradation.
The situation is especially worrying in most developing countries in particular
those of Sub-Saharan Africa due to the absence of regulation or control. The
present work aims to isolate bacterial strains able to degrade hydrocarbons
which can later be used in biotechnology for environments depollution.
Oil-contaminated wastewater samples were collected in Ouagadougou city (Burkina
Faso) and then used as source of bacterial isolation. Appropriate amounts of
samples were inoculated to a mineral salt medium (MS) with Total Quartz 9000
oil as sole carbon and energy source and then incubated for enrichment, prior
to microbe isolation. Two bacterial strains namely S2 and S7 were isolated from the enrichment cultures. The strains were tested for their
ability to degrade other hydrocarbons (i.e. gasoline, diesel oil, brake oil) and for temperature, pH and salt concentration
ranges for growth before their biochemical characteristics were defined. Based
on their morphological, physiological and biochemical traits, strains S2 and S7 belong to Acinetobacter and Pseudomonas genera,
respectively.