TITLE:
A Polymerase Chain Reaction Based DNA Fingerprinting Technique: Amplified Fragment Length Polymorphism for Molecular Typing of Perchlorate Reducing Bacteria
AUTHORS:
Anita Shete, Vikram Ghole, Manju Raina, Bikash Aich
KEYWORDS:
AFLP—Amplified Fragment Length Polymorphism, Arthrobacter, Ammonium Perchlorate, Bio-Degradation
JOURNAL NAME:
Journal of Environmental Protection,
Vol.5 No.12,
September
23,
2014
ABSTRACT: Genetic profiling
of environmentally important organisms is very essential for easy identification
of biodegrading bacteria. In the previous study, we have reported the
perchlorate biodegrading bacteria and characterized them by biochemical
analysis and 16 S sequencing. We have observed a very similar isolates of
Arthrobacter (Actinobacteria) degrading 4.1 mM and 4.7 mM of ammonium
perchlorate [1]
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. In this study, we report PCR based DNA fingerprinting technique to
generate the genomic signature of these closely related group of Arthrobacter
species. This study also effectively generates unique genomic signature for
each of these isolates that has potential for use in molecular monitoring as
well as for tracking genomic variation and rearrangements.