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Maligaya, M.L., Chan, C.A., Jacobson, J.D., Patton, W.C., Corselli, J. and Chan, P.J. (2006) A Follow-Up Expanded Study of the Correlation of Sperm Velocity in Seminal Plasma and Offspring Gender. Archives of Andrology, 52, 39- 44.

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• TITLE: Variation among Beef Bulls in the Ratio of X- to Y-Chromosome Bearing Spermatozoa

  AUTHORS: Rick W. Rorie, Pedro A. Delgado, Toby D. Lester

  KEYWORDS: Sex Ratio, Spermatozoa, Ejaculates, Semen, Bulls

  JOURNAL NAME: Advances in Reproductive Sciences, Vol.2 No.4, September 22, 2014

  ABSTRACT: A study was conducted to evaluate variation in the ratio of X- to Y-bearing sperm of individual ejaculates and to determine any relationship between skewed sex ratio and either routine morphological evaluation or computer assisted sperm analysis (CASA). Semen was collected from bulls weekly for 6 consecutive weeks, sperm DNA was recovered and quantitative, and real-time PCR was used to determine the ratio of X- to Y-bearing sperm in each ejaculate. The overall mean of X-bearing sperm within ejaculates was 54.7% over the 6 weeks of semen collections. The percentages of X-bearing sperm were similar (P > 0.5) across all collections. Between bulls, there were differences (P