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Hamalainen, M., Nieminen, R., Vuorela, P., Heinonen, M. and Moilanen, E. (2007) Anti-Inflammatory Effects of Flavonoids: Genistein, Kaempferol, Quercetin, and Daidzein Inhibit STAT-1 and NF-κB Activations, Whereas Flavone, Isorhamnetin, Naringenin, and Pelargonidin Inhibit Only NF-κB Activation along with Their Inhibitory Effect on iNOS Expression and NO Production in Activated Macrophages. Mediators of Inflammation, 2007, 45673-45683.

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• TITLE: Qualitative and Quantitative Analysis of Five Bioactive Flavonoids in Salix bordensis Turcz. by HPLC-DAD and HPLC-ESI-MS

  AUTHORS: Lei Zhao, Lina Liu, Jintian Li

  KEYWORDS: Flavonoids, Salix Bordensis Turcz., Qualitative and Qualitative Analysis, High-Performance Liquid Chromatography-Diode Array Detection, High-Performance Liquid-Chromatography-Electrospray Tandem Mass Spectrometry

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.5 No.13, September 19, 2014

  ABSTRACT: The qualitative characterization and quantitative analysis of five bioactive flavonoids in Salix bordensis Turcz. were achieved via reversed-phase high-performance liquid chromatography coupled with diode array detection and tandem mass spectrometry, by using an Agilent ZORBAX SB-C18 HPLC column with a gradient elution of 0.3% (v/v) formic acid in water and methanol as the mobile phase. The compounds in the mixture were clearly identified by comparing their HPLC-DAD ultraviolet spectra, retention times, and MS data with those of corresponding reference compounds. All calibration curves showed good linearity (r2 > 0.9998) within the test ranges. The LOD, LOQ, specificity, precision, and accuracy for the method were validated. The results demonstrated that this analytical approach is ideal for the determination of bioactive compounds, such as flavonoids, and that it constructed a basis for the comprehensive evaluation of the quality of Salix bordensis Turcz.