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International Organization for Standardization (1994) ISO 5725-3. Accuracy (Trueness and Precision) of Measurement Methods and Results—Part 3: Intermediate Measures of the Precision of a Standard Measurement Method. ISO/TC 69 Applications of Statistical Methods. Geneva.

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• TITLE: Adaptation and Validation of a Proteolytic Activity Methodology Using Dye-Crosslinked Substrate Tablets

  AUTHORS: Fernanda Martins, Elaine C. Ramires

  KEYWORDS: UV-Vis Spectrophotometry, Protease, Protazyme, Enzymatic Cleaner, Azurine-Crosslinked Casein, Colorimetry

  JOURNAL NAME: Advances in Enzyme Research, Vol.2 No.3, September 12, 2014

  ABSTRACT: Enzymatic activities are important to be quantified in products as enzymatic cleaners, which are used in medical and surgical devices reprocessing. Enzymatic activities are critical for the proper chemical cleaning that intends to remove solid organic dirt from inaccessible sites. The most important enzyme for this purpose is the protease, which is able to dissolve the main dirt attached to medical and surgical instruments. In this context, this study contributes to the development of a new proteolytic activity quantification method and its validation. The methodology is based on colorimetry and uses a UV-Vis spectrophotometer to measure the substrate hydrolysis by the blue color intensity, employing Protazyme AK tablets as substrate.