TITLE:
Directly immobilized DNA sensor for label-free detection of herpes virus
AUTHORS:
Phuong Dinh Tam, Mai Anh Tuan, Nguyen Duc Chien
KEYWORDS:
DNA Sensor; Hybridization; APTS
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.2 No.6,
October
26,
2009
ABSTRACT: This paper reports the direct immobilization of deoxyribonucleic acid (DNA) sequences of Herpes simplex virus (5’–AT CAC CGA CCC GGA GAG GGA C–3’) on the surface of DNA sensor by using the cyclic voltammetric method with the presence of pyrrole. The potential was scanned from –0.7 volt to + 0.6 volt, the scanning rate was at 100mV/s. This kind of DNA sensor was developed to detect Herpes virus DNA in real samples. The FTIR was applied to verify specific binding of DNA sequence and conducting polymer, the morphology of conducting polymer doped with DNA strands was investigated by using a field emission scanning electron microscope (FE-SEM). The results showed that output signal given by coimmobilized DNA/PPy membrane sensor was better than that given by APTS immobilized membrane sensors. The sensor can detect as low as 2 nM of DNA target in real samples.