TITLE:
Transcript Level Analysis of Lignin and Flavonoid Biosynthesis Related Genes in Eucalyptus globulus
AUTHORS:
Tomotaka Shinya, Kazunori Hayashi, Shinichi Onogi, Akiyoshi Kawaoka
KEYWORDS:
HCT, CHS, 4CL, Transcript Level, Lignin Content, Eucalyptus globulus
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.18,
August
26,
2014
ABSTRACT: We have investigated a correlation of transcript abundances of key genes
that influence the quality of wood and flavonoid biosynthesis, such as the two p-hydroxycinnamoyl-CoA:quinate
shikimate p-hydroxycinnamoyl transferase (HCT) and the two chalcone synthases (CHS) from Eucalyptus globulus grown in a greenhouse. The EglHCT1 and EglHCT2
transcripts accumulated in stems of all ages, but to a lesser extent in leaves.
On the other hand, EglCHS3
and EglCHS4 exhibited
high transcript levels in leaves, roots and shoots, but low levels in the stem.
A positive correlation (R2 > 0.70) was observed between the transcript
levels of the EglHCT1, EglHCT2 genes and Klason lignin (KL)
content. In addition, the sum of transcript levels of EglHCT1 and EglHCT2 genes were highly correlated to KL
contents (R2 > 0.85). However, there is no relationship between
transcript levels of two CHS genes
and, KL or flavonoid contents. This may imply that lignin biosynthesis and flavonoid
biosynthesis are independently regulated in E. globulus.