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Konimani, K., Nakabayshi, J., Nagai, T., Tsujimura, Y., Chiba, K., Kimura, H., Miyawaki, A., Sawasaki, T., Yokota, H., Manabe, N. and Sakamaki, K. (2012) The Molecular Mechanisms of Caspase-8 Activation: Quantitative Experimental Validation of a Mathematical Model. Biochimica et Biophysica Acta-Molecular Cell Research, 182, 1825-1840.

has been cited by the following article:

• TITLE: NF-κB Controls Resistance of Human Salivary Gland (HSG) Cells to Apoptosis in an in Vitro Model of Sjogren’s Syndrome

  AUTHORS: Yan Wang, Syed A. Jamal, Luis F. Torres-Romero, Agostino Molteni, Alexander Shnyra, Carole McArthur

  KEYWORDS: Acinar, Ductal, Cytokines, Salivary Glands, Sjogren’s Syndrome, Tumor Necrosis Factor-Alpha

  JOURNAL NAME: Open Journal of Rheumatology and Autoimmune Diseases, Vol.4 No.3, August 26, 2014

  ABSTRACT: Aim: To elucidate the anti-apoptotic properties of nuclear factor kappa light-chain-enhancer of activated B cells (NF-κB) and feedback regulation of NF-κB by nuclear factor of kappa light-chain-enhancer of activated B-cells inhibitor alpha (IκBα). Methods: We developed an in vitro model of Sjogren’s syndrome by transfecting human salivary gland (HSG) and acinar cells (NS-SV-AC) with a plasmid-encoding IκBαM (pCMV-IκBαM), a degradation-resistant IκBα (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha)-mutant, and examined TNF-induced apoptosis and anti-apoptotic properties of NF-κB. Apoptosis and induction of pro-apoptotic and anti-apoptotic genes were investigated by cDNA arrays, RT-PCR, electrophoretic mobility shift assays, and western blot. Results: In the presence of NF-κB inhibitors, TNF-induced apoptosis was markedly increased in both salivary gland and acinar cells. Increased caspase-3 activity was present in both HSG and NS-SV-AC cells. IκBαM-transfected salivary gland cells were more sensitive to TNF-induced apoptosis than IκBαM-transfected acinar cells. Transcription of pro-apoptotic genes was confirmed in both HSG and NS-SV-AC cells that were transfected with IκBαM. Results from caspase-3 activity assay confirmed previous experiments showing an apoptotic role for NF-κB. Conclusion: Data from gene expression arrays suggest that different mechanisms may operate during TNF-induced apoptosis in salivary gland ductal and acinar cells.