TITLE:
Chronic Toxicity Study in Rats Orally Exposed to Mulberry Sea-Buckthorn Beverage Concentrate
AUTHORS:
Qingping Sun, Zhimin Xu
KEYWORDS:
Chinese Traditional Medicine, Chronic Toxicity Test, Mulberry Sea-Buckthorn Beverage Concentrate, Rats
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.5 No.9,
August
22,
2014
ABSTRACT: Objective: This
study is designed to observe the chronic toxicity after the administration of
mulberry sea-buckthorn beverage concentrate for 3 months on rats and to predict
the possible adverse effect and the potential toxicity target organs. Method:
The rats (SPF level) were randomly divided into high-dose (20 mL/kg BW),
middle-dose (10 mL/kg BW), low-dose (5 mL/kg BW) groups and negative control
group (20 mL/kg BW of purified water) with 30 rats in each group. Each group
was orally given mulberry sea-buckthorn beverage concentrate for 3 months and recovered
by stop feeding samples for 2 weeks for a recovery observation. The rats’
general condition, the organ coefficient, the indexes of hematology and blood biochemistry
and the histological changes of the main organs were determined. Result: The
appearance and behavior of activity in rats showed no anomalies in all these
groups and all the rats put on weight during this period. Comparing to the negative
control group, no obvious differences were observed in the weekly weight and
organ coefficient of each dose group. After 3 months of administration, HGB in
both mulberry sea-buckthorn beverage concentrate low-dose group and high-dose
group were increased. No significant differences were observed in the indexes
of hematology after 2 weeks of recovery. CREA in low-dose, middle-dose and
high-dose groups were significantly increased after 3 months of administration
and it remained in the high level in middle-dose and high-dose group even after
2 weeks of recovery. No drug-related lesions were observed in the histological
changes of major organs. Conclusion: The results show that long term use of
mulberry concentrated sea-buckthorn beverage can lead to increased CREA, which suggested
kidney toxicity. Although no obvious pathological change was found in kidney, we
should pay attention to chronic kidney damage in the further research.