TITLE:
Effects of Light Emitting Diode in Erythrocytes
AUTHORS:
Hennes Gentil de Araújo, Patricia Froes Meyer, Mario Bernardo Filho, Éric Heleno Freire Frederico, Sebastião David dos Santos Filho, Marcus Vinicius de Mello Pinto
KEYWORDS:
LED, Blood, Spectrophotometer
JOURNAL NAME:
Modern Research in Inflammation,
Vol.3 No.3,
August
22,
2014
ABSTRACT: The Light Emitting Diode
(LED) is a phototherapy equipment, poorly studied, which is able of acting on
blood level and it has frequently appeared in clinical practice of
physiotherapy to aid on skin rejuvenation, control inflammation and wound
healing. The aim of the study was to evaluate the effects of LED on red blood
cells through the spectrophotometer. This is an experimental study with a
sample of 5 male Wistar rats weighting between 200 g and 350 g, randomly
selected, anesthetized and 4 ml of blood was collected by cardiac puncture. The
blood collected was divided into four groups, one control and three treated,
these individually irradiated by blue LED (420 - 490 nm), green LED (515 - 570
nm) and red LED (620 - 680 nm) at an output of 3 watts, for 10 minutes and 5 cm
apart from the surface of the blood, then it was analyzed by a
spectrophotometer at 540 nm. The results were submitted to Anova and Post-roch
Turkey (p produced
hemolysis greater than the control in hypotonic solutions of NaCl (range 0.02
to 0.06 M) (p p