TITLE:
Detection of Contamination and Analysis of Vertical Transmission of BmNPV in Eggs and Moths of Bombyx mori
AUTHORS:
Cláudia Regina das Neves Saez, Roxelle Ethienne Ferreira Munhoz, Naiara Climas Pereira, Thaís Souto Bignotto, Verônica Aureliana Fassina, Graziele Milani Pessini, Laura Beatriz Garay, Lucinéia de Fátima Chasko Ribeiro, Rose Meire Costa Brancalhão, Maria Aparecida Fernandez
KEYWORDS:
Silkworm Diseases, Sericulture, Viruses in Insects, BmNPV, Bombyx mori
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.5,
August
22,
2014
ABSTRACT: This study reports the
molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian
Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx
mori female moths of each strain, followed by PCR amplification. A pair of
primers was designed based on a specific sequence of the baculovirus genome
related to the BmNPV ORF 14. Another pair of primers was used to amplify the
silkworm Actin A3 gene segment, which was used as positive control. Twenty gene
pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp),
which indicated the presence of the BmNPV. The frequency of contaminated moths
was as following: 100% for silkworm strains M18-2, M12-2 and J1; 83% for C25,
C75 and C24 strains; 66% for KR01; 50% for M11-A; 33% for AS3, B106, M8 and M11
and 16% for C211, E8 and Hindu strains. These are promising results for the
identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent
generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the
detection of the fragments of the expected size (443 bp). The difficulty in
detecting BmNPV contamination in B. mori eggs
may be due to the low concentration of virus in samples.