TITLE:
Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide
AUTHORS:
Sanders Junglee, Laurent Urban, Huguette Sallanon, Félicie Lopez-Lauri
KEYWORDS:
Hydrogen Peroxide, Potassium Iodide, One Step, Microplate, Inexpensive
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.5 No.11,
August
21,
2014
ABSTRACT: Here, we present an optimization of colorimetric determination of hydrogen peroxide content in plants using potassium iodide. Our method is based on a one step buffer (extraction and reaction) for the determination of H2O2 in different plant tissues and overcomes interference of soluble antioxidant and color background. A particular attention is paid to buffer pH shown to be tissue dependent. With this inexpensive microplate method, it is possible to analyze 12 experimental samples in about 45 min all in triplicates, with blanks, controls and standard curve.