TITLE:
Establishment and Optimization Growth of Shoot Buds-Derived Callus and Suspension Cell Cultures of Kaempferia parviflora
AUTHORS:
Ab Rahman Zuraida, Othman Ayu Nazreena, Kamarulzaman Fatin Liyana Izzati, Ahmad Aziz
KEYWORDS:
Kaempferia parviflora, Suspension Cultures, Meristematic Shoots, Callus Induction
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.18,
August
18,
2014
ABSTRACT: Callus and
suspension cells culture of Kaempferia
parviflora was successfully established. Meristematic shoots can be used
for utilization of plant cell biosynthetic capabilities for obtaining useful
products from valuable medicinal plant to meet out the pharmaceutical demand
and also for studying the metabolism. The medium containing combination of 0.2
mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.2 mg/L napthyleneacetic
acid (NAA) promoted the highest callus induction at 20%. Transferring the
initiated callus on the medium with 1 mg/L 2,4-D enhanced the proliferation
rate up to maximum fresh weight of 6.71 gm. Growth curve of cultured cells
revealed that the cells continued to grow until 50 days of culture and showed
the highest peak (fresh weight) at 40 days in all different initial weight
tested ( 0.2, 0.5 and 1.0 gram). Isolated embryogenic callus was found to
produce the highest in weight when suspended in liquid medium supplemented with
1 mg/L 2,4-D at 110 rpm resulted 13.5 gram fresh weight and 1080 mg dry weight.