TITLE:
Global Proteomics of the Extremophile Black Fungus Cryomyces antarcticus Using 2D-Electrophoresis
AUTHORS:
Kristina Zakharova, Katja Sterflinger, Ebrahim Razzazi-Fazeli, Katharina Noebauer, Gorji Marzban
KEYWORDS:
Microcolonial Fungi, Extremophiles, Proteomics, Protein Characterization
JOURNAL NAME:
Natural Science,
Vol.6 No.12,
August
18,
2014
ABSTRACT: The
microcolonial black fungus Cryomyces
antarcticus is an extremophile organism growing on and in rock in the
Antarctic desert. Ecological plasticity and stress tolerance make it a perfect
model organism for astrobiology. 2D-gel electrophoresis and MALDI-TOF/TOF mass
spectrometry were performed to explore the protein repertoire, which allows the
fungus to survive in the harsh environment. Only a limited number of proteins
could be identified by using sequence homologies in public databases. Due to
the rather low identification rate by sequence homology, this study reveals
that a major part of the proteome of C.
antarcticus varies significantly from other fungal species.