TITLE:
Isolation and Characterization of 26 Microsatellite Loci for the Shortfin Silverside Fish Chirostoma humboldtianum Valenciennes1835 (Atherinopsidae: Menidiinae) Derived from Next Generation Sequencing and Their Cross-Amplification in Central Mexican Atherinopsids
AUTHORS:
Barriga-Sosa Irene de los Angeles, Rosa María García-Martínez, Jhoana Díaz-Larrea, Oscar Adrián Lozano Garza, Francisco Javier García-De-León
KEYWORDS:
Endemic, Polymorphism, Villa Victoria, Poblana alchichica, C. promelas, C. riojai
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.5,
August
18,
2014
ABSTRACT: The endemic silverside fish C. humboldtianum is of great
ichtyologycal, economical and cultural relevance in central Mexico and it has
been suggested that it is among a group of other “peces blancos”, the most
ancestral species. Here we characterized a set of 26 microsatellite loci from
the species in order to further assess population and phylogeographic issues
that aid in evaluating their highly impacted populations. From 58 primer pairs
tested on specimens from Villa Victoria dam (Rio Balsas Basin), 26 loci
positively amplified on the species and cross-primed on specimens of the
closely related and threatened Poblana
alchichica, C. promelas and C. riojai. Loci resolved medium and high
genetic variation (mean NA = 14.3, SD = 9.38; mean HO & HE = 0.47, SD = 0.32
and 0.58, SD = 0.32, respectively) and it is expected that these polymorphic
loci are also useful in studing threatened atherinopsid species.