TITLE:
Molecular Characterization on the Early Developmental Stages of the Egyptian Toad Bufo regularis Reuss
AUTHORS:
Alaa El-Din H. Sayed, Afaf I. Elballouz, Ekbal T. Wassif
KEYWORDS:
Genes, Bufo regularis, Protein, DNAs
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.5,
August
15,
2014
ABSTRACT: The present study
aimed to investigate changes in the protein contents using SDS-PAGE, also using
the random amplified polymorphic DNA (RAPD-PCR) assay to identify changes in
DNA concentration in the embryos of Egyptian toad Bufo regularis. Our experiment
started when tadpoles began to feed. The adapted embryos were divided into 3
large tanks of 200 embryos each, collections of samples started from feeding
age every three days. Cluster method was used to indicate the distinct distance
between the band patterns of different stages. An increase occurred in the
concentration of protein fractions in these tadpoles upon metamorphosis and
that when metamorphosis was completed. DNA concentration exhibited an evident
low values at the premetamorphic stage 44 (105.85 ± 0.519 ng/μl) compared with
the other more advanced developmental stages 55 and 56 (204.056 ± 0.651 ng/μl
and 234.55 ± 2.325 ng/μl respectively). In conclusion, acquire information
about the changes in the molecular content of Bufo regularis during
metamorphosis has been estimated.