TITLE:
Molecular Characterization of Peruvian Fowl Adenovirus (FAdV) Isolates
AUTHORS:
Jorge Rodríguez, Ysabel Koga, Arnaldo Alvarado, Robert Tinoco
KEYWORDS:
Fowl Adenovirus C, Molecular Characterization, Inclusion Body Hepatitis, Peru
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.10,
August
6,
2014
ABSTRACT: Forty seven clinical samples of
Fowl adenovirus (FAdV) associated with Inclusion Body Hepatitis (IBH) from
Peruvian broilers received between July 2006 and April 2013 were genotyped
using sequencing of L1 Loop of Hexon gene. All 47 clinical samples presented
macroscopic and histopathology lesions consistent with IBH, and amplified a
specific fragment of Hexon gene by Polymerase Chain Reaction (PCR). A unique
nucleotide sequence of 789 base pairs of Hexon gene (position 273 to 1061) was
obtained in all 47 clinical samples analyzed. This sequence showed a high level
of conservation in amino acid and nucleotide sequence (>99%) with a Fowl
Adenovirus C serotype 4 previously identified. Sequence and phylogenetic
analysis indicate no genotypic variation in Peruvian isolates. The presence of
a unique genotype very closely related with genotype C1 previously reported in
Peru and Ecuador (>99%), suggests the presence of FAdV C serotype 4 genotype
C1 in clinical cases of IBH from Peruvian broilers.