TITLE:
Product Containing “Skin Calm Complex” Improves Barrier Effect in Vitro
AUTHORS:
María Matabuena-de Yzaguirre, Gabriela Bacchini, Eva Vila-Martínez, Ángeles Juarranz
KEYWORDS:
Atopic Dermatitis, Epidermal Barrier, Epithelial Regeneration and Differentiation
JOURNAL NAME:
Journal of Cosmetics, Dermatological Sciences and Applications,
Vol.4 No.4,
August
5,
2014
ABSTRACT: Epidermal Barrier
integrity is deteriorated in many skin conditions, including Atopic Dermatitis,
and could be responsible for many of the associated clinical symptoms. The aim
of this study was to determine the effect of REPAVAR ATOPIC SKIN BODY CREAM EXTREME,
product containing “SKIN CALM COMPLEX” on barrier epidermis structure.
Morphologic and structural evaluations were conducted on reconstructed human
epidermis, by qualitative (hematoxylin/eosin- and immune-staining) and
quantitative (MTT assay) techniques. Immature (Age Day 10) and mature (Day 17)
sample tissues were treated with the product for 24 hours or 7 days,
respectively. Morphological and quantitative analysis revealed an increase in
the thickness of the epidermis in both cases after the treatment. The study by
immunostaining showed that Product-treated tissues presented excellent
localization of: 1) E-cadherin in keratinocytes of the spinous layer; 2)
Filaggrin and Loricrin, in the granular layer; and 3) increased expression of
Involucrin and Keratin 10 in suprabasal layers and 4) elevated expression of
Keratin 5 in the basal layer of the epidermis. The product REPAVAR ATOPIC SKIN
BODY CREAM EXTREME increases the epithelial regeneration and differentiation
determined by cell proliferation and the expression and localization of
specific proteins, improving the epidermal barrier effect.