TITLE:
Diversity of Secondary Metabolites from Two Antarctic Microbes Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011
AUTHORS:
Cheng Wang, Xiaoqing Tian, Qiao Yang, Yanan Lu, Liyan Ma, Hongliang Huang, Chengqi Fan
KEYWORDS:
Antarctic Microbes, Rhodococcus sp. NJ-008, Pseudomonas sp. NJ-011, Secondary Metabolite
JOURNAL NAME:
Open Journal of Marine Science,
Vol.4 No.3,
July
31,
2014
ABSTRACT: Diversity analysis on
secondary metabolites of Antarctic microbes, Rhodococcus sp. NJ-008 and Pseudomonas sp. NJ-011, together with the structural elucidation of some purified compounds,
has been carried out for understanding of their chemical constituents. The
methanol extracts of Rhodococcus sp.
NJ-008 and Pseudomonas sp. NJ-011 were
subjected to HPLC-TOF MS test for diversity analysis on secondary metabolites,
respectively. The chemical constituents of NJ-011 are mainly N-containing
compounds including some alkaloids and short polypeptides, while those of
NJ-008 are not N-containing ones. Three compounds were also isolated and
identified from extract of NJ011 by different column chromatography and
preparative HPLC, and their structures were elucidated as b-carboline (1),
3-benzylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione (2) and 3-isobutylhexahydropyrrolo[1,2-a]pyrazine-1,4-dione
(3) by comparison of TOF MS, 1Hand 13C-NMR data with
those reported. More microbial material of Pseudomonas sp. NJ-011 should be needed for exploration of the minor constituents with
complicated structures.