TITLE:
Polymerase Chain Reaction Detection of ASFV Infection in Nigerian Indigenous Pig
AUTHORS:
Olufunke O. Oluwole, Galman O. Omitogun
KEYWORDS:
Nigerian Indigenous Pig, Polymerase Chain Reaction, African Swine Fever
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.4 No.3,
July
31,
2014
ABSTRACT: This work
investigated the presence of African Swine Fever Virus (ASFV) in Nigerian
Indigenous Pig (NIP) using Polymerase Chain Reaction (PCR) screening method on
the extracted DNA from the pig blood. NIP population selected from south
western Nigeria were used for this study. ASFV infected blood samples collected
from the University of Ibadan were used as positive control. Adiscrete and
specific band was observed in both NIP and the infected samples show the
presence of ASFV in NIP. The bands were of the expected size 278 base pairs (bp).
The implication of this finding is that NIPs have domiciled ASFV without
showing any clinical symptoms.