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Chen, S., Yee, A., Griffiths, M., Larkin, C., Yamahiro, C.T., Behari, R., Paszko-Kolva, C., et al. (1997) The Evaluation of a Fluorogenic Polymerase Chain Reaction Assay for the Detection of Salmonella Species in Food Commodities. International Journal of Food Microbiology, 35, 239-250.
http://dx.doi.org/10.1016/S0168-1605(97)01241-5

has been cited by the following article:

• TITLE: Development of a Duplex Real-Time PCR Method for the Pharmaceutical Rapid Microbial Detection of Staphylococcus aureus and Pseudomonas aeruginosa

  AUTHORS: Tiehao Lin, Liying Lin, Pu Zeng

  KEYWORDS: S. aureus, P. aeruginosa, Duplex Real-Time PCR, Pharmaceutical

  JOURNAL NAME: Journal of Biosciences and Medicines, Vol.2 No.5, July 22, 2014

  ABSTRACT: Objective: To develop a duplex real-time PCR assay for pharmaceutical rapid microbial detection of Staphylococcus aureus and Pseudomonas aeruginosa. Methods: The specific primers and probes were designed to amplify the femB gene of S. aureus and the DNA gyrase subunit B gene of P. aeruginosa. The sensitivity of the system was detected by a multiple proportional dilution method. In order to examine the specificity of the system, other twenty-one bacteria strains were assayed simultaneously. Results: A highly sensitive and specific duplex real-time PCR assay for the detection of S. aureus and P. aeruginosa was established. The sensitivity was 50 copies/μL. The specificity was 100%. The whole detection procedure can be finished within 2.5 h. Conclusion: The duplex real-time PCR method is efficient in detecting with good sensitivity and specificity. There is a good prospect of this method applying in disease prevention and pharmaceutical industry due to the simultaneous detection of two pathogens.