TITLE:
Effects of Lithium and Valproic Acid on the Production of Brain-Derived Neurotrophic Factor in Astrocytoma
AUTHORS:
Koji Ohtomo, Satoshi Nishino, Masatake Kurita, Hirobumi Mashiko, Shin-ichi Niwa
KEYWORDS:
BDNF, Bipolar Disorder, Lithium, PLCγ, TrkB, Valproic Acid
JOURNAL NAME:
Open Journal of Psychiatry,
Vol.4 No.3,
July
22,
2014
ABSTRACT: No systematic
investigation considering BDNF and its receptor TrkB has been conducted on the
effects of mood-stabilizing drugs. We therefore decided to quantify BDNF
production and the expression of TrkB-T1 receptor and PLCγ in astrocytoma. 1321N human astrocytoma cells were grown to a
sufficient quantity in 5% fetal calf serum (FCS). The mood-stabilizing drugs Li
and VPA were added to the therapeutic concentrations of 1 mM and 600 μM,
respectively. The production of BDNF was determined by an enzyme-linked
immunosorbent assay. The expressions of TrkB-T1 and PLCγ were determined by Western blot. The production of BDNF was
significantly higher on Day 7 in the VPA samples (P γ expression did not change. Li and VPA
seem differently affect BDNF production and TrkB-T1 (BDNF receptor) expression.