TITLE:
Heat-Resistant Properties of α-Chymotrypsin Adsorbed onto Biomass Charcoal Powder
AUTHORS:
Hidetaka Noritomi, Shunichi Kurihara, Nobuyuki Endo, Satoru Kato
KEYWORDS:
Adsorption, Biomass Charcoal Powder, α-Chymotrypsin, Activity, Thermal Stability
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.5 No.3,
July
21,
2014
ABSTRACT: Biomass charcoal powder (BCP) was used as a
carrier for immobilization of α-chymotrypsin
through adsorption. BCP was derived from plant biomass wastes such as dumped
bamboos by oxygen-free pyrolysis at low temperatures and grinding with a jet
mill. The activity of adsorbed α-chymotrypsin
was strongly dependent upon the kind of BCP. The thermal denaturation curve of
adsorbed α-chymotrypsin was shifted
to high temperature, compared to that of free one. When α-chymotrypsin adsorbed onto BCP of bamboos was incubated at 45°C, the half-life of adsorbed α-chymotrypsin was 2.6 times greater
than that of free one. After incubation at 45°C, the remaining activity of adsorbed α-chymotrypsin markedly depended on the
kind of BCP.