TITLE:
Study of the Effect of Silymarin on Viability of Breast Cancer Cell Lines
AUTHORS:
Saieh Hajighasemlou, Mohammadmorad Farajollahi, Mahmoud Alebouyeh, Hossein Rastegar, Mojgan Taghizadeh Manzari, Milad Mirmoghtadaei, Behjat Moayedi, Maryam Ahmadzadeh, Mansure Kazemi, Farzad Parvizpour, Safoora Gharibzadeh
KEYWORDS:
Silymarin, Breast Cancer, SK-BR-3, BT-474
JOURNAL NAME:
Advances in Breast Cancer Research,
Vol.3 No.3,
July
21,
2014
ABSTRACT: Background: Breast cancer is the most
prevalent cancer and results in 14% of cancer-related deaths among women
worldwide. The aim of this study is to investigate the anticancer effects of
Silymarin on two breast cancer cell lines (BT-474, SK-BR-3). Methods and
Material: Two breast cancer cell lines—SK-BR-3 and BT-474—were incubated for 24
hours in standard conditions before adding 100, 200, 400, 800, 1600 μM
Silymarin to each well. Alamar blue was then added to the wells after 24, 48
and 72 hours of incubation and cell viability was determined using fluorescence
reader to detect the optical density. Results were analyzed using generalized
estimating equations (GEE) method in STATA 12.0. Results: we demonstrated theSilybum
marianuminhibition of two-cell lines SK-BR-3 and BT-474 growth at
different concentrations after 24, 48 and 72 hours. Silymarin increased cell
death in both cell lines. Conclusion: Silymarin can be combined with other
anti-neoplastic agents to obtain better results.