TITLE:
Absence of Expression of OCT-2 in Gastric Cancer Cells
AUTHORS:
José Paulo Freire, Maria Emilia Oliveira, Maria Conceição Crujo, Ana Rute Fernandes, José Crespo Mendes de Almeida, Henrique Bicha Castelo
KEYWORDS:
Gastric Cancer, Lymph Node Metastasis, OCT-2, Stem Cells
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.4 No.3,
July
16,
2014
ABSTRACT: OCT-2, a member of the POU homeodomain family of transcription factors,
has been implicated in gastric cancer lymph node metastasis on a single
publication so far. Other members of the same family of transcription factors
were found to have a role in intestinal metaplasia and gastric cancer (OCT-1)
and in trophectoderm differentiation of embryonic stem cells (OCT3/4) via
modulation ofCDX2. These findings
prompted us to evaluate whether OCT-2 could in fact have a role in gastric
oncogenesis and lymph node metastasis in human gastric cancer, designing an immunohistochemistry
study of the putative expression of OCT-2 on human gastric cancer tissue of 69
surgical archival specimens of intestinal and diffuse type. We could not find
expression of OCT-2 on any of our cancer tissues, metaplasia or normal gastric
mucosa despite the expression of OCT-2 on tonsil lymphatic cells (tissue test)
and normal lymph nodes. We concluded that, based on immunohistochemistry, OCT-2
does not have a role in gastric oncogenesis nor does have any relationship with
lymph node metastasis in gastric cancer.