TITLE:
Leptin Causes the Early Inhibition of Glycolysis- and TCA Cycle-Related Genes in the Brain of Ob/Ob Mice to Restore Fertility
AUTHORS:
Carlos Fernandes Baptista, Samuel Marcos Ribeiro de Noronha, Maria de Nazareth Gamboa Ritto, Eduardo Henrique da Silva Freitas, Melquíades Pereira Júnior, Mauro Abi Haidar, Ismael Dale Cotrim Guerreiro da Silva, Silvana Aparecida Alves Corrêa de Noronha, Marisa Teresinha Patriarca
KEYWORDS:
PCOS, Obesity, Leptin, Glycolysis, TCA Cycle, Gene Expression
JOURNAL NAME:
American Journal of Molecular Biology,
Vol.4 No.3,
July
16,
2014
ABSTRACT: Introduction: Polycystic
ovarian syndrome (PCOS) is undoubtedly the commonest androgen disorder in
woman’s fertile period and certainly one of the most prevalent causes of
anovulation. The syndrome has an estimated prevalence of 4% - 10% among women
of childbearing age. Previously, our group demonstrated the effect of gonadal
white adipose tissue transplantation from wild-type lean and fertile female
mice to isogenic obese anovulatory ob/ob mice. These complex metabolic
interrelationships between obesity and PCOS have yet to be fully understood.
The aim of this study was to evaluate the effect of gonadal white adipose
tissue (WAT) transplantation from the wild-type lean and fertile female mice to
isogenic obese, anovulatory mice (Lep ob/Lep ob) on the expression of
glycolysis- and TCA cycle-related genes and obtain a general view of the
glucose metabolism in the brain of these animals. Methods: Fifteen ob/ob mice
ranging from 2 to 3 months of age were divided into 3 experimental groups:
control normal weight (n = 5), obese control (n = 5) and obese 7 days leptin
treated (n = 5). The whole brains of the mice were processed for RNA
extraction. The samples from each group were used to perform PCR assays using
an array plate containing 84 primers to study the glucose metabolism-related
genes. Results: The glycolysis- and TCA cycle-related genes were significantly downregulated.
The most significantly affected genes were as follows: for glycolysis (fold
regulation with p