TITLE:
Spore Types and Spore Production of Ramularia areola for Screening Cotton Germplasm for Resistance
AUTHORS:
Jéssica Volponi, Janaina N. Matos, Larissa Girotto, Mariana S. Marangoni, Rafael Galbieri, Yeshwant R. Mehta
KEYWORDS:
Gossypium hirsutum, Ramularia Leaf Blight, Techniques, Fruiting Bodies
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.15,
July
16,
2014
ABSTRACT: Spore production ofRamularia areolahas always been a
difficult task. Brazilian isolates ofR.
areolaproduce spores of variable size and shape. The typical spores are 3
septate, rarely 4 and 5 septate, together with abundant single celled oblong to
round bodies—a phenomenon not reported earlier forR. areola. Budding of spores is a continuous process as observed in
our isolates. By repeated culturing the pathogen either ceases to produce typical
spores or loses its pathogenic character. To solve this problem, a technique
was developed to produce large quantity of typical spores under laboratory
conditions. Sporulating cultures produced on Petri plates containing V8
juice-agar were kept on the laboratory bench till they became dry and then
stored at 5°C for reisolation and/or for production of fresh inoculum. In such
dried cultures spores remain viable for a period of over 12 months, and hence
isolates ofR. areolaoriginatedfrom different geographic regions can
be maintained in sporulating form. Results of the present investigation would
aid cotton breeders and pathologists in screening germplasm resistant toRamulariaand in other genetical studies
under glasshouse conditions.