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Pezenti, L.F., Barbosa, J., Mariane, A.V., Mariana, S.M., Jessica, V., Almeida, W.P., Galbieri, R. and Mehta, Y.R. (2013) Phenotypic Variability among Isolates of Ramularia areola from Brazilian Cotton. Tropical Plant Pathology, 38, 329-331.
http://dx.doi.org/10.1590/S1982-56762013005000023

has been cited by the following article:

• TITLE: Spore Types and Spore Production of Ramularia areola for Screening Cotton Germplasm for Resistance

  AUTHORS: Jéssica Volponi, Janaina N. Matos, Larissa Girotto, Mariana S. Marangoni, Rafael Galbieri, Yeshwant R. Mehta

  KEYWORDS: Gossypium hirsutum, Ramularia Leaf Blight, Techniques, Fruiting Bodies

  JOURNAL NAME: American Journal of Plant Sciences, Vol.5 No.15, July 16, 2014

  ABSTRACT: Spore production ofRamularia areolahas always been a difficult task. Brazilian isolates ofR. areolaproduce spores of variable size and shape. The typical spores are 3 septate, rarely 4 and 5 septate, together with abundant single celled oblong to round bodies—a phenomenon not reported earlier forR. areola. Budding of spores is a continuous process as observed in our isolates. By repeated culturing the pathogen either ceases to produce typical spores or loses its pathogenic character. To solve this problem, a technique was developed to produce large quantity of typical spores under laboratory conditions. Sporulating cultures produced on Petri plates containing V8 juice-agar were kept on the laboratory bench till they became dry and then stored at 5°C for reisolation and/or for production of fresh inoculum. In such dried cultures spores remain viable for a period of over 12 months, and hence isolates ofR. areolaoriginatedfrom different geographic regions can be maintained in sporulating form. Results of the present investigation would aid cotton breeders and pathologists in screening germplasm resistant toRamulariaand in other genetical studies under glasshouse conditions.