TITLE:
Sugarcane Transcript Profiling Assessed by cDNA-AFLP Analysis during the Interaction with Sugarcane Mosaic Virus
AUTHORS:
Cibele N. F. Medeiros, Marcos C. Gonçalves, Ricardo Harakava, Silvana Creste, Paula M. Nóbile, Luciana R. Pinto, Dilermando Perecin, Marcos G. A. Landell
KEYWORDS:
Plant Resistance, Plant Viruses, Molecular Markers, Candidate Genes, cDNA-AFLP, SCMV
JOURNAL NAME:
Advances in Microbiology,
Vol.4 No.9,
July
11,
2014
ABSTRACT: Sugarcane mosaic
caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present
study, changes in the transcription profile obtained by cDNA-AFLP analysis were
investigated in two sugarcane varieties contrasting to SCMV resistance, when
challenged with a severe virus strain. Healthy plants derived from meristem tip
tissue culture were mechanically inoculated under greenhouse controlled
conditions and sampled at 24, 48 and 72 hours after inoculation. A total of 392
transcript-derived fragments (TDFs) were verified in the resistant variety
against 380 in the susceptible one. The two sugarcane genotypes showed differential
behavior in the number of induced and repressed TDFs along the time-course
samplings. Ten out of 23 sequenced TDFs (unique from the resistance variety),
showed identity with known plant sequences, mostly related to plant defense
mechanisms against pathogens. The cDNA-AFLP technique was effective in
revealing changes in the transcription profile within and between contrasting
varieties when challenged by SCMV.