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Munazir, M., Qureshi, R., Ali, M., Rashid, U., Noor, S., Mehmood, K., Ali, S. and Arshad, M. (2010) Primary Callus Induction, Somatic Embryogenesis and Regeneration Studies in Elite Wheat Varieties from Pakistan. Pakistan Journal of Botany, 42, 3957-3965.

has been cited by the following article:

• TITLE: Somatic Embryogenesis and Organogenesis for Regeneration of Endangered Multipurpose Desert Plant Leptadenia pyrotechnica Forsk. Decne in the Kingdom of Bahrain

  AUTHORS: Manal Ahmed Sadeq, Malabika Roy Pathak, Ahmed Ali Salih, Mohammed Abido, Asma Abahussain

  KEYWORDS: Endangered Plants, Ex Situ Conservation, Leptadenia pyrotechnica, Plant Regeneration, Organogenesis, Somatic Embryogenesis

  JOURNAL NAME: American Journal of Plant Sciences, Vol.5 No.15, July 10, 2014

  ABSTRACT: Leptadenia pyrotechnica is an important multipurpose endangered plant in the Kingdom of Bahrain with restricted distribution. Nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of indole acetic acid (IAA) and 6-benzylaminopurine (BAP). Initially, 80% and 60% explants responded in direct shoot and callus initiation response respectively in presence of 8.88 μM BAP with 5.71 μM IAA in modified MS media after two weeks of culture. The highest frequency of plant regeneration was observed in presence of 8.88 μM BAP with 1.14 μM IAA following organogenic pathway of differentiation. Hundred percent callus proliferation was observed while initial callus developed in presence of 4.44 μM BAP with 2.85 μM IAA and was transferred in media containing 4.44 μM, 6.66 μM BAP with 2.85 μM IAA and 13.32 μM BAP with 5.71 μM IAA. The callus derived plants were regenerated following the pathway of indirect somatic embryogenesis. The induction of somatic embryogenesis and plant regeneration from callus was also observed in modified MS media supplemented with 4.44 μM BAP and 2.85 μM IAA. The plant regeneration protocol we developed for Leptadenia pyrotechnica will be very beneficial for biodiversity conservation and environment protection of Bahrain. Moreover, the present paper reports for the first time specifically the somatic embryogenesis in this multipurpose desert plant Leptadenia pyrotechnica.