TITLE:
Lidocaine-Induced Cell Growth of Human Gingival Fibroblasts. Role of Na+-K+-ATPase and PKC Activities
AUTHORS:
Emmanuel Quinteros Villarruel, Betina Orman, Enri Borda
KEYWORDS:
Lidocaine, DNA Synthesis, Human Gingival Fibroblasts, Na+-K+-ATPase, PKC
JOURNAL NAME:
Pharmacology & Pharmacy,
Vol.5 No.8,
July
10,
2014
ABSTRACT: Background:
Evidences have shown that local anaesthetics are clinically useful compounds that
exert a pharmacological effect by blocking nerve impulse propagation and also it
is able to provoke proliferation and cell growth. Aims: The aim of this study was to investigate the proliferation and
cell growth capacity of lidocaine on human gingival fibroblast cells and the different
signal pathways involved in its effect. Method:
For this purpose in vitro cultures of human gingival fibroblasts
were assayed and the effects of lidocaine on proliferation and cell DNA synthesis,
Na+-K+-ATPase and PKC activities and K+ efflux
were also evaluated. Results: Lidocaine
stimulated in a concentration-dependent manner proliferation and cell growth of
human gingival cells and the mechanism involve an increment in Na+-K+-ATPase
and PKC activities, which led to an increase in K+ release. All of these
effects were blocked by tetrodotoxin, ouabain and calphostin C. In addition, PMA
(activator of PKC) increased per se the DNA synthesis of human gingival fibroblast
cells. Conclusions: This work demonstrates
that lidocaine increase human gingival fibroblasts DNA synthesis and proliferation
through an activation of PKC pathway accompanied by the stimulation of Na+-K+-ATPase
activity with an increase in K+ efflux. These results contribute to showing
another action of lidocaine different to its general use as a drug that relieves
odontologic pain or acts as an anti-arrithmogenic agent.