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Sawamoto, K., Nakao, N., Kakishita, K., Ogawa, Y., Toyama, Y., Yamamoto, A., Yamaguchi, M., Mori, K., Goldman, S.A., Itakura, T. and Okano, H. (2001) Generation of Dopaminergic Neurons in the Adult Brain from Mesencephalic Precursor Cells Labeled with a Nestin-GFP Transgene. Journal of Neuroscience, 21, 3895-3903.

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• TITLE: Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery

  AUTHORS: Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza

  KEYWORDS: Stem Cells Recovery, CD133+, Percoll, Density Gradients

  JOURNAL NAME: Stem Cell Discovery, Vol.4 No.3, July 3, 2014

  ABSTRACT: Circulating CD133+ stem cells from the peripheral blood have been shown to be able to differentiate into numerous cell lineages. However, adults have only a small number of these circulating stem cells. The aim of the present study was to assess a new isolation and enrichment technique for CD133+ stem cells from peripheral blood with the use of Percoll density gradients. Our results demonstrated the presence of two large mononuclear bands when whole blood was centrifuged with 48% and 50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major CD133+ enrichment at the 48% Percoll concentration in one of the two bands. Further culture of these cells resulted in the formation of multiple colony-forming units. Our results suggest an advantage from using a simple Percoll gradient for successful CD133+ cell recovery, which could aid in differentiation and transplantation protocols.