TITLE:
Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery
AUTHORS:
Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza
KEYWORDS:
Stem Cells Recovery, CD133+, Percoll, Density Gradients
JOURNAL NAME:
Stem Cell Discovery,
Vol.4 No.3,
July
3,
2014
ABSTRACT: Circulating CD133+ stem
cells from the peripheral blood have been shown to be able to differentiate
into numerous cell lineages. However, adults have only a small number of these
circulating stem cells. The aim of the present study was to assess a new
isolation and enrichment technique for CD133+ stem cells from peripheral blood
with the use of Percoll density gradients. Our results demonstrated the presence
of two large mononuclear bands when whole blood was centrifuged with 48% and
50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major
CD133+ enrichment at the 48% Percoll concentration in one of the two bands.
Further culture of these cells resulted in the formation of multiple
colony-forming units. Our results suggest an advantage from using a simple
Percoll gradient for successful CD133+ cell recovery, which could aid in
differentiation and transplantation protocols.