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Matsumoto, K., Yasui, K., Yamashita, N., Horie, Y., Yamada, T., Tani, Y., Shibata, H. and Nakano, T. (2000) In Vitro Proliferation Potential of AC133 Positive Cells in Peripheral Blood. Stem Cells, 18, 196-203. http://dx.doi.org/10.1634/stemcells.18-3-196

has been cited by the following article:

• TITLE: Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery

  AUTHORS: Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza

  KEYWORDS: Stem Cells Recovery, CD133+, Percoll, Density Gradients

  JOURNAL NAME: Stem Cell Discovery, Vol.4 No.3, July 3, 2014

  ABSTRACT: Circulating CD133+ stem cells from the peripheral blood have been shown to be able to differentiate into numerous cell lineages. However, adults have only a small number of these circulating stem cells. The aim of the present study was to assess a new isolation and enrichment technique for CD133+ stem cells from peripheral blood with the use of Percoll density gradients. Our results demonstrated the presence of two large mononuclear bands when whole blood was centrifuged with 48% and 50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major CD133+ enrichment at the 48% Percoll concentration in one of the two bands. Further culture of these cells resulted in the formation of multiple colony-forming units. Our results suggest an advantage from using a simple Percoll gradient for successful CD133+ cell recovery, which could aid in differentiation and transplantation protocols.