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Zvaifler, N.J., Marinova-Mutafchieva, L., Adams, G., Edwards, C.J., Moss, J., Burger, J.A. and Maini, R.N. (2002) Mesenchymal Precursor Cells in the Blood Of Normal Individuals. Arthritis Research, 2, 477-488. http://dx.doi.org/10.1186/ar130

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• TITLE: Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery

  AUTHORS: Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza

  KEYWORDS: Stem Cells Recovery, CD133+, Percoll, Density Gradients

  JOURNAL NAME: Stem Cell Discovery, Vol.4 No.3, July 3, 2014

  ABSTRACT: Circulating CD133+ stem cells from the peripheral blood have been shown to be able to differentiate into numerous cell lineages. However, adults have only a small number of these circulating stem cells. The aim of the present study was to assess a new isolation and enrichment technique for CD133+ stem cells from peripheral blood with the use of Percoll density gradients. Our results demonstrated the presence of two large mononuclear bands when whole blood was centrifuged with 48% and 50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major CD133+ enrichment at the 48% Percoll concentration in one of the two bands. Further culture of these cells resulted in the formation of multiple colony-forming units. Our results suggest an advantage from using a simple Percoll gradient for successful CD133+ cell recovery, which could aid in differentiation and transplantation protocols.