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Hofstetter, C.P., Schwarz, E.J., Hess, D., Widenfalk, J., El Manira, A., Prockop, D.J. and Olson, L. (2002) Marrow Stromal Cells Form Guiding Strands in the Injured Spinal Cord and Promote Recovery. Proceedings of the National Academy of Sciences of the United States of America, 99, 2199-2204.
http://dx.doi.org/10.1073/pnas.042678299

has been cited by the following article:

• TITLE: Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery

  AUTHORS: Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza

  KEYWORDS: Stem Cells Recovery, CD133+, Percoll, Density Gradients

  JOURNAL NAME: Stem Cell Discovery, Vol.4 No.3, July 3, 2014

  ABSTRACT: Circulating CD133+ stem cells from the peripheral blood have been shown to be able to differentiate into numerous cell lineages. However, adults have only a small number of these circulating stem cells. The aim of the present study was to assess a new isolation and enrichment technique for CD133+ stem cells from peripheral blood with the use of Percoll density gradients. Our results demonstrated the presence of two large mononuclear bands when whole blood was centrifuged with 48% and 50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major CD133+ enrichment at the 48% Percoll concentration in one of the two bands. Further culture of these cells resulted in the formation of multiple colony-forming units. Our results suggest an advantage from using a simple Percoll gradient for successful CD133+ cell recovery, which could aid in differentiation and transplantation protocols.