TITLE:
Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma
AUTHORS:
Hanen Chammem, Imen Hafaid, Olivier Meilhac, Farid Menaa, Laurence Mora, Adnane Abdelghani
KEYWORDS:
C-Reactive Protein, Protein G, Surface Plasmon Resonance Imaging, Human Plasma
JOURNAL NAME:
Journal of Biomaterials and Nanobiotechnology,
Vol.5 No.3,
July
2,
2014
ABSTRACT: In this work, we describe
an approach of detecting biomarkers by Surface Plasmon Resonance imaging (SPRi)
technique in real samples. Two C-Reactive Protein (CRP)-antibody immobilization
methods were used: The first method was based on direct physisorption of
CRP-antibody onto gold surface; the second one was based on oriented
CRP-antibody with protein G intermediate layer. The two developed immunosensors
were tested against CRP antigen in phosphate buffer saline solution with the
SPRi technique. The response of the developed immunosensors was reproducible
and stable. The detection limit of 10 pg·mLǃ and 50 pg·mLǃ CRP-antigen was observed with and without protein G respectively with this
technique. Moreover, the developed SPRi immunosensor was used for CRP-antigen
detection in human plasma. A detection limit of 5 ng·mLǃ and 10 ng·mLǃ was obtained with and without protein G respectively. These obtained results
were compared to those obtained with QCM (Quartz Crystal Microbalance) and
Enzyme-Linked Immunosorbent Assay (ELISA) techniques.