TITLE:
Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC Coupled with Mass Spectrometry
AUTHORS:
Olga S. Brushinina, Roman V. Gurto, Maksim S. Timofeev, Galina A. Frelikh, Vladimir A. Slepichev, Elena A. Yanovskaya, Natalia U. Polomeeva, Yulya G. Zyuz’kova, Vladimir V. Udut
KEYWORDS:
Candesartan, Hydrochlorothiazide, LC-MS, Pharmacokinetic
JOURNAL NAME:
International Journal of Analytical Mass Spectrometry and Chromatography,
Vol.2 No.2,
June
30,
2014
ABSTRACT: Quantitative determination of hydrochlorothiazide (HCTZ) and candesartan (CDS) in human plasma in volunteers was performed using a sensitive, selective and specific LC-MS method which has been developed and validated before the study. The study was performed by means of a liquid chromatograph Shimadzu Prominence equipped with a mass spectrometer LCMS-2020. Analytical column PerfectBond ODS-HD HPLC-column 5 μm 250 × 3.0 mm with a pre-column cartridge PerfectBond ODS-HD 5 μm 10 × 3.0 mm, double source of ionization for LCMS-2020 (electrospray (ESI) and chemical (APCI)) and software LabSol LCMS V5 LCMS2020 systempack were used. The low limit of the quantitative determination for HCTZ and CDS made up 10 ng/ml. m/z for CDS 441.20—positive scan, m/z for HCTZ 295.90—negative scan. The method has been applied to a pharmacokinetic study of 12.5 mg HCTZ and 16 mg CDS tablet in healthy volunteers.