TITLE:
Analysis of Linkage for Ten X-STR Markers in a Rio de Janeiro (Brazil) Three-Generation Family Sample
AUTHORS:
Roberto Chan, Elizeu Fagundes de Carvalho, Juliana Gozi de Aquino, Dayse Aparecida da Silva, Gisele Lôbo-Hajdu
KEYWORDS:
X-Chromosome STR Markers, Recombination, Probability of Linkage, LOD Score
JOURNAL NAME:
Open Journal of Genetics,
Vol.4 No.3,
June
27,
2014
ABSTRACT: Recently, typing of
polymorphisms on the X chromosome has become a standard technique in forensic
genetics and a growing number of short tandem repeats (STR) has been
established in this chromosome related to genetic population studies. Knowledge
of marker recombination is very important especially when the X chromosome
typing is used in forensic kinship analysis. It is known that the meiotic
recombination is not a simple function of the physical distance between
segments of the DNA but the recombination events between them tend to be
clustered at special regions of the chromosome. Information on the rate of
recombination among markers can be gathered by studying families through
several generations. In this work we have typed DNA samples of pedigree
consisting of nineteen families in Rio de Janeiro, constituted of grandfather,
mother and grandson, and in some cases grandmother and aunt, and reported the
recombination of 10 STR markers of the X chromosome. The study of the linkage
analysis using the LOD score has shown that the marker pairs DXS8378-DXS7423,
DXS7132-DXS9898, DXS7132-GATA172D05 DXS9898-DXS7133 and DXS6809-DXS7133 are not
transmitted in a random way, during a recombination event.