TITLE:
Morpho-Histological Analysis of Direct Shoot Organogenesis Induced in Flower Buds Cultures of Allium altissimum
AUTHORS:
Tatyana V. Poluboyarova, Tatyana I. Novikova, Galina Yu. Vinogradova, Elena V. Andronova
KEYWORDS:
Allium altissimum, Direct Shoot Organogenesis, Regeneration, Tissue Culture
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.5 No.13,
June
24,
2014
ABSTRACT: The present work
gives a detailed study of in vitro shoot organogenesis of the ornamental onion A.
altissimum Regel from the buds of the middle layer of the inflorescences.
The course of morphogenesis was examined by light and scanning electron
microscopy. Histological observation revealed that during 3 - 5 days of culture
on the BDS medium supplemented with 2.0 mg·L-1 of
BA and 2.0 mg·L-1 of NAA the
epidermal cells of the stamen filament in the area of its fusion with the tepal
became competent and dedifferentiated. Originally the organogenesis involved
several initial epidermal cells. The formation of meristematic centers was
observed from day 3 to day 14. The apical shoot meristems and leaf primordia in
a roller shape formed from day 14 to day 28 of culture on the same media. The
further development of vegetative shoots and formation of the bulblets were
observed when the explants were stimulated by triapenthenol (2.0 mg·L-1).